Peptide Reconstitution Guide

What You'll Need

• Lyophilized peptide vial - Your sealed peptide
• Bacteriostatic water (BAC water) - Contains 0.9% benzyl alcohol preservative
• Insulin syringes - For measuring and transferring
• Alcohol swabs - For sterilizing vial tops
• Clean work surface - Sanitized area free of contaminants

Why Bacteriostatic Water?

Bacteriostatic water is preferred over sterile water because:

• Preservative effect: Benzyl alcohol inhibits bacterial growth
• Longer shelf life: Reconstituted peptides last 4-6 weeks refrigerated
• Multiple uses: Can draw from vial multiple times safely

Step-by-Step Reconstitution Process

Step 1: Prepare Your Workspace

• Clean and sanitize your work surface
• Wash hands thoroughly or wear gloves
• Gather all supplies before starting
• Ensure peptide vial has reached room temperature (if refrigerated)

Step 2: Calculate Water Volume

Decide how much water to add based on desired concentration. Common approaches:

Step 3: Sterilize Vial Tops

• Wipe the top of the peptide vial with an alcohol swab
• Wipe the top of the BAC water vial with an alcohol swab
• Allow alcohol to dry completely (30 seconds)

Step 4: Draw Bacteriostatic Water

• Remove cap from a new insulin syringe
• Insert needle into BAC water vial through rubber stopper
• Invert vial and draw desired amount of water
• Remove any air bubbles by tapping syringe
• Withdraw needle from BAC water vial

Step 5: Add Water to Peptide

This is the most critical step.

• Insert needle through peptide vial rubber stopper
• Aim needle at the inside wall of the vial (not directly at the powder)
• Slowly release water, letting it run down the side of the vial
• Do NOT spray directly onto the lyophilized powder
• Do NOT shake or agitate vigorously

Step 6: Allow to Dissolve

• Let the vial sit for 1-2 minutes
• If needed, gently swirl (don't shake) to help dissolution
• Most peptides dissolve within minutes
• Solution should be clear when fully dissolved

Step 7: Inspect the Solution

After reconstitution, the solution should be:

• ✅ Clear and colorless (most peptides)
• ✅ Free of visible particles
• ✅ Completely dissolved (no powder remaining)

Do not use if:

• ❌ Solution is cloudy or discolored
• ❌ Visible particles floating
• ❌ Peptide won't dissolve after 10+ minutes of gentle swirling

Step 8: Store Properly

• Immediately refrigerate reconstituted peptide (2-8°C / 36-46°F)
• Keep away from light
• Use within 4-6 weeks for BAC water reconstitution
• Label vial with reconstitution date and concentration

Reconstitution Tips

Slow is Better

Take your time adding water. Rushing increases the chance of damaging the peptide or introducing contamination.

Temperature Matters

Let refrigerated peptides warm to room temperature before reconstituting. Cold peptides may not dissolve as easily.

Don't Over-Concentrate

While using less water creates higher concentrations, some peptides have solubility limits. If the peptide won't dissolve completely, add more water.

Use Fresh Supplies

Always use new, sterile syringes. Never reuse needles or syringes, even between draws from the same vial.

Common Reconstitution Problems

Peptide Won't Dissolve

Possible causes:

• Water added too cold - let warm and try gentle swirling
• Not enough water - add more BAC water
• Degraded peptide - may have been damaged in shipping/storage

Solution Becomes Cloudy

Possible causes:

• Contamination - discard and use new supplies
• Incompatible diluent - ensure you're using appropriate water
• Peptide degradation - may indicate damaged product

Air Bubbles in Vial

Small bubbles are normal and won't affect the peptide. They will dissipate over time. Avoid injecting air into the vial when adding water.

Water Volume Reference

Common reconstitution volumes for easy dosing: