Ipamorelin: Research Profile and Purity Standards

Ipamorelin: Research Profile and Purity Standards

Molecular Structure

Ipamorelin is a pentapeptide composed of five amino acids with the sequence Aib-His-D-2-Nal-D-Phe-Lys-NH2. It belongs to the class of growth hormone secretagogues (GHS) which specifically target the ghrelin receptor, also known as the growth hormone secretagogue receptor (GHS-R). The molecular formula for Ipamorelin is C38H49N9O5, and it has a molecular weight of 711.852 g/mol. Its structural stability and specificity make it a popular choice in research focused on growth hormone-related studies.

Mechanism of Action

Ipamorelin functions by mimicking ghrelin, the endogenous ligand of the GHS-R, leading to a significant increase in growth hormone (GH) secretion from the anterior pituitary gland. Unlike other GHS, Ipamorelin does not significantly affect cortisol or prolactin levels, providing a more selective action. This selectivity is attributed to its specific binding affinity to the GHS-R1a receptor subtype. Researchers have found that Ipamorelin stimulates GH release without the concomitant release of ACTH, making it a cleaner and more targeted option for studies involving GH modulation.

Research Applications

Ipamorelin has garnered attention in various research domains, including endocrinology, metabolism, and aging. It is particularly studied for its potential in muscle wasting conditions, growth deficiencies, and its effects on bone density. Moreover, Ipamorelin's ability to promote lean body mass and its minimal side effects compared to other GHS make it a viable candidate for investigating therapeutic approaches in age-related degeneration and catabolic states.

Quality Markers to Look For

Ensuring the quality of Ipamorelin is crucial for obtaining reliable research results. Key quality markers include:

• Purity: High-performance liquid chromatography (HPLC) analysis should show a purity of ?98% for research-grade Ipamorelin.
• Identity Confirmation: Mass spectrometry should confirm the molecular weight of 711.85 g/mol.
• Solvent Content: Karl Fischer titration may be used to verify water content is below 5%.
• Peptide Content: Verified by amino acid analysis to ensure batch consistency.

Common Impurities

Several impurities can arise during peptide synthesis, and detecting them ensures the compound's efficacy and safety in research applications. Common impurities in Ipamorelin include:

• Truncated peptides: Result from incomplete synthesis reactions and should be identified through MALDI-TOF analysis.
• Oxidative derivatives: Occur due to oxidation of methionine or tryptophan residues, best detected by LC-MS.
• Racemization: D-amino acid residues formed during synthesis, affecting peptide activity.

Storage Requirements

Proper storage of Ipamorelin is essential to maintain its stability and activity:

• Lyophilized Form: Should be stored at -20°C in a desiccated environment to prevent moisture uptake.
• Reconstituted Form: Store at 2-8°C and use within 48 hours to prevent degradation.
• Avoid Repeated Freeze-Thaw Cycles: To prevent peptide breakdown, aliquot appropriately to minimize thawing frequency.

Practical Tips for Researchers

For those sourcing Ipamorelin for research purposes, consider the following tips:

• Verify supplier credentials and check for ISO certifications to ensure high manufacturing standards.
• Request a comprehensive Certificate of Analysis (CoA) including HPLC and MS data.
• Consider custom synthesis options for specific modifications or labeling needs.
• Use inert gas packaging to prevent oxidation during transportation and storage.

Key Takeaways

• Ipamorelin is a selective GHS with specific action on GH release without increasing cortisol or prolactin.
• Purity standards require ?98% purity as determined by HPLC.
• Common impurities include truncated peptides and oxidative derivatives, which should be monitored using advanced analytical methods.
• Proper storage involves maintaining lyophilized Ipamorelin at -20°C and avoiding repeated freeze-thaw cycles.
• Choose reputable suppliers and verify quality through detailed CoA and analytical data.

By adhering to these guidelines, researchers can ensure the acquisition and use of high-quality Ipamorelin, facilitating accurate and meaningful research outcomes.